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浓度为50ng/uL, 容量为20uL/支
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文献和实验1 A260 unit of duplex DNA = 50 ug 1 A260 unit of single-stranded DNA = 37 ug 1 A260 unit of single-stranded RNA = 50 ug 1 kb of DNA = 333 amino acids of coding capacity = 37,000 daltons Densities (50% GC
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
TRANSFORMATION BY VACUUM INFILTRATION This protocol is modified from Bechtold, Ellis and Pelletier (1993). "In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants". [C.R. Acad. Sci. Paris, Life Sciences 316: 1194-1199
Comprehensive High‐Throughput Arrays for Relative Methylation (CHARM)
) 100 mM (100×) guanosine triphosphate (GTP; store in single‐use aliquots at −80°C) 10 U/µL McrBC (New England Biolabs): test each new lot on a standard gDNA sample
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