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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 供应商:
东莞汉诺生物
- 英文名:
Marking Dye Kit for Tissue - Seven Color
可将标本边缘着色,标记标本的方向。这种标记可以是固定后的标本,尤其适合标皮肤癌标本。
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
background. 4. Incubate tissue in X-Phos/NBT Detection Buffer for 15' at room temperature. XPhos/NBT Detection Buffer (Buffer 3 as described for Genius kit by Boehringer-Mannheim) 100 mM Tris-HCl, pH 9.5 100 mM NaCl 50 mM MgCl
with a fluorescent dye. Probes are labeled with one of the four fluorescent dyes. The color of a fluorescent dye indicates 4 of 16 possible dinucleotides in the 1st and 2nd position of the probe. A random probe is ligated to a phosphorylated primer annealed
of ProLong kit according to manufacturer's specifications (Molecular Probes) or use an equivalent method. About 25 µl of medium is needed per cover slip.Steps 1. Hybridization is tested before color-coding and multiple transcript detection. We started
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