BstF5I内切酶北京厂家

特别提示：包括BstF5I内切酶在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：BstF5I内切酶
英文名称：BstF5I
产品货号：GS2077
产品规格：250U

储存条件：冷冻（-20℃）

使用说明

The minimum number of units that resulted in complete digestion of 1μgof substrate DNA in 16hoursis 0.25.

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 65℃ in a total reaction volume of 50μl.
Recognition sites	GGATGNN↑
来源	Bacillus stearothermophilus F5
Assayed on	Lambda DNA
Optimal buffer	Y(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.)
Optimal temperature	65℃
Storage conditions	10 mM Tris-HCl(pH 7.5);50 mM KCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;and 50%glycerol;Store at -20℃.
Ligations	After 10-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 20 u.a.of enzyme for 16hoursat 65℃.
Methlation effect	Methylation sensitivity not tested
Thermal inactivation	enzyme is inactivated by incubation at 80℃ for 20 mins.
Campatible Ends	BseGI,BtsCI,FokI^

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