CCL4 encoded by the CCL4 gene,also known as Macrophage Inflammatory Protein-1β(MIP-1β)is a CC chemokine with specificity for CCR5 receptors and it is a major HIV-suppressive factor produced by CD8+ T cells.It is a monokine with inflammatory and chemokinetic properties.Recombinant CCL4 induces a dose-dependent inhibition of different strains of HIV-1,HIV-2,and simian immunodeficiency virus(SIV).Specifically,MIP-1-beta(3-69)is also a ligand for CCR1 and CCR2 isoform B.Recombinant human CCL4 contains 69 amino acids and it shares 77%and 80%a.a.sequence identity with murine and rat CCL4,respectively.Both human and murine MIP-1α and MIP-1β are active on human and murine hematopoietic cells.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
外观
Sterile Filtered White lyophilized(freeze-dried)powder.
纯度
>96%by SDS-PAGE and HPLC analyses.
来源
Escherichia coli.
生物活性
Fully biologically active when compared to standard.The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 5.0-20ng/ml.
成分
Lyophilized from a 0.2μmfiltered concentrated solution in 20mMPB,pH 7.4,150 mM NaCl.
内毒素
Less than 1 EU/ug of rHuMIP-1β/CCL4 as determined by LAL method.
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20?C.Further dilutions should be made in appropriate buffered solutions.