Traut’s Reagent(2-Iminothiolane or 2-IT)is a cyclic thioimidate compound for thiolation(sulfhydryl addition).Traut’sReagent
reacts with primary amines(-NH2)to introduce sulfhydryl(-SH)groups while maintaining charge properties similar to the
original amino group(Figure 1).Once added,sulfhydryl groups may be specifically targeted for reaction in a variety of
useful labeling,cross-linking,and immobilization procedures.