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文献和实验ELISPOT (Enzyme-linked ImmunoSPOT)
ELISPOT: Left: Human PBMCs cultured for 24 hrs (no mitogen). Right: Human PBMCs activated with PMA/Ionomycin for 24 hrs Human Granzyme B ELISPOT: Left: Human PBMCs without mitogen cultured for 24 hrs. Right: Human
A Cell-Detachment Solution Can Reduce Background Staining in the ELISPOT Assay
-lβ, IL-2, IL-4, IL-5, and IL-8 and mouse IL-4, IL-6, and TNF-α ELISPOT assays, allowing one to remove a large number of adhered cells without hindering ELISPOT assay performance. However, Accumax was incompatible with human IFN-γ, mouse IFN-γ, canine
Dual-Color ELISPOT Assay for Analyzing Cytokine Balance
A dual-color enzyme-linked immunospot (ELISPOT) assay enabled us to analyze three kinds of cytokine-secreting cells simultaneously. T helper (Th) cells can be subdivided into at least two distinct functional subsets based on their cytokine
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