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T7无细胞表达载体
Thermo Scientific™ T7无细胞表达载体(pT7CFE1)是一种克隆质粒,专门针对Thermo Scientific一步法人体外蛋白表达系统进行了优化。这款质粒可用于基因的体外翻译(IVT),结合一步法人IVT系统时可翻译得到功能蛋白质。pT7CFE1可在N端或C端添加单亲和标签或串联亲和标签,有助于蛋白质纯化及检测等。
T7无细胞表达载体订购信息
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文献和实验Overview of Vector Design for Mammalian Gene Expression
, and; 4. Many proteins are readily secreted from mammalian cells providing the ability to isolate the protein from conditioned medium that contains low amounts of protein when cells are propagated under serum-free conditions.
Dual-Expression Vectors for Efficient Protein Expression in Both E. coli and Mammalian Cells
functions in the cell. Dual-expression vectors for expression of proteins encoded by these genes in mammalian and bacterial cells can be used for this characterization. Typically, eukaryotic genes are expressed in mammalian cells to characterize biological
Use of a Retroviral Vector for Expression of a Foreign Gene
. Viral vectors, including retroviruses, adenoviruses, and the Sinbis virus have been developed over the last decade for foreign gene expression in mammalian cells. This chapter will discuss the use of a replication-incompetent retroviral vector in cell
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