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文献和实验【共享】如何检测GFP、GFP、YFP、EYFP、CFP抗体?
检测GFP、EGFP、YFP、EYFP、CFP的GFP抗体GFP是绿色萤光蛋白(Green Fluorescent Protein)的简称,由238个氨基酸残基组成。GFP蛋白质最早是由下村脩等人在1962年在一种学名Aequorea victoria的水母中发现。其基因所产生的蛋白质,在蓝色波长范围的光线激发下,会发出绿色萤光。GFP或其突变体EGFP标签系统等被广泛用于基因表达效率的检测,以及和目的蛋白融合表达用于检测目的蛋白的表达和分布。我们都知道GFP抗体能应用于GFP的表达检测、纯化
Visualization of the Expression of Green Fluorescent Protein (GFP)-Linked Proteins
of the jellyfish, Aequoria victoria (1 , 2 ). The original expression plasmid for this jellyfish protein, green fluorescent protein (GFP) yielded a low level of fluorescence. However, through mutagenesis, enhanced GFPs have been produced that are about 35 times
Green Fluorescent Protein (GFP) as an Indicator of Transfection in Chicken Embryos
Green fluorescent protein (GFP) is responsible for the bioluminescence of the Pacific Northwest jellyfish, Aequorea victoria. In A.victoria, the 27-kDa protein absorbs blue light from a photoprotein that is activated by calcium and emits green
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