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文献和实验Protein A Purification of Antibody
1. Reagents(1) Affi-gel Protein-A Agarose (BioRad #153-6153)(2) MAPS II Binding Buffer (BioRad # 153-6161)(3) 0.314 g/ml diH2O(4) MAPS II Elution Buffer (BioRad #153-6162)(5) 0.023 g/ml diH2O(6) MAPS II Regeneration Buffer (BioRad #153-6166(7) 1M
Antibody Purification: Affinity Chromatography Protein A and Protein G Sepharose
not only to isolate antigen-specific antibodies but also to remove specific contaminants from biological samples. Methods are described for the purification of immunoglobulins, namely IgG, IgG fragments and subclasses, using the high affinity of protein A and protein
Protein A Purification of Antibody
Reagents Affi-gel Protein-A Agarose (BioRad #153-6153) MAPS II Binding Buffer (BioRad # 153-6161) 0.314 g/ml diH2 O MAPS II Elution Buffer (BioRad #153-6162)
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