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文献和实验Carbohydrate-Specific Adhesion of Intact Cells to Resolved Glycolipids on TLC Plates
on ice 150mm Petri dishes Glutaraldehyde X-ray film (or alternate cell detection system) Protocol: 1. Prescored 10 x 10 cm HPTLC plates are broken along one score to generate a 5 cm x 10 cm
Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
apparatus. Aspirate with pasteur pipets as before. A triple wash is required, this time using the Nunc washer to fill and aspirate each row 3x simultaneously before moving on to the next row. Dry the wells by removing the remaining liquid with pasteur pipets
Dual- and Triple-Color Fluorospot
in sterile filtered (0.2 μm) PBS (Mabtech). 实验设备 1. Transparent, flat-bottomed, 96-well plates with polyvinylidene difluoride (PVDF) membranes designed for low autofluorescence (IPFL; Millipore, Cork, Ireland). 2. Analysis
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