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文献和实验In vitro mutagenesis using Altered Sites
DNA does not affect mutagenesis. Ⅱ.Phosphorylation of Oligo 1.Resuspend 1ug of oligo in 17.5 μl dH2O. Add 2.0μl of 10x kinase buffer and 5U T4 polynucleotide kinase. Incubate at 37℃ for 30 minutes. 10x kinase buffer: 500mM Tris-Cl pH7.5 100mM
PCR Genotyping of Embryos and Blastocysts
. The final concentration of PK being anywhere from 60-400 ug/ml. This is then heated to anywhere from 50-65°C for 30-60 min. then the sample is heated to 95°C or boiled to inactivate the PK. A small volume of this lysate is then processed normally for PCR
MATERIALS T-cell: CD2 IgG2b, 01171D B-cell: B220 (CD45R) IgG2a, 01121A CD3 IgG2b, 28001D Monocytic: Mac1 (CD11b) IgG2b, 01711A CD5 IgG2a, 01031
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