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文献和实验An Alternative Fixative to Formalin Fixation for Molecular Applications: The RCL2-CS100 Approach
specimen. Recently, we found RCL2� -CS100, a noncross-linking fixative, to display interesting performances regarding tissue morphology and DNA, RNA, and protein quality. We adapted RCL2 tissue fixation protocol so it could be used on a routine
Methanol Fixation for Immunofluorescence
. We recommend that you try both types of fixation for your particular antibody/fusion protein when working out your initial immunofluorescence conditions. MeS Buffer 100 mM MeS, pH 6.9 1 mM EGTA 1 mM MgCl2 Store at 4°C Methanol Fix (100
Zymolyase Dissolve in 10% glucose. Store aliquots (25 µl) at 20o C in common freezer for stock solutions Use once and dispose Comments: alternative is to dissolve in SCE, 40% gycerol, 20 mg/ml final. Can use
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