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- Gibco™
- 10565018
- 2026年01月16日
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- 详细信息
- 文献和实验
- 技术资料
- 规格：
500ml
Description
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.This DMEM / F-12 is modified as follows:
| With | Without |
| • GlutaMAX™ | • HEPES |
| • Phenol Red |
The complete formulation is available.
Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2environment to maintain physiological pH.
cGMP manufacturing and quality system
DMEM/F-12 is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical DMEM/F-12 product made in our Scotland facility (31331-028). This facility is also registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standard.
Specifications
| Glutamine: | GlutaMAX™-I |
|---|---|
| Phenol Red Indicator: | Phenol Red |
| Form: | Liquid |
| HEPES Buffer: | No HEPES |
| Sodium Pyruvate Additive: | Sodium Pyruvate |
| Serum Supplementation: | Standard Serum Supplementation |
| Glucose: | High Glucose |
| Product Line: | Gibco®, GlutaMAX™ |
| Product Size: | 500 mL |
| Volume (Metric): | 500 ml |
| Green Features: | Sustainable packaging |
| Shipping Condition: | Room Temperature |
Contents & storage
Storage conditions: 2-8° C. Protect from lightShipping conditions: Ambient
Shelf life: 12 months from date of manufacture
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文献和实验Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
Embryonic Fibroblasts (Irradiated) 4. DMEM with GlutaMAX™-I (High Glucose) 5. KnockOut™ DMEM/F-12 6. Fetal Bovine Serum (FBS), ES Cell-Qualified 7. KnockOut™ Serum Replacement (KSR) 8. MEM Non-Essential Amino Acids (NEAA)
无血清培养的首选是AIM V培养基（SFM）。在开始进行新的细胞培养时，可以参考下表所列的条件： 细胞系 细胞类型 种 组织 推荐使用的培养基 293 成纤维细胞 人 胚胎肾 MEM, 10% 热灭活马血清 3T6 成纤维细胞 小鼠 胚胎 DMEM, 10% 胎牛血清 A549 上皮细胞 人 肺癌 F-12K, 10%胎牛血清 A9 成纤维细胞 小鼠 结缔组织 DMEM, 10%胎牛血清 AtT-20 上皮细胞 小鼠 垂体
Differentiating Neural Stem Cells into Neurons and Glial Cells
实验试剂 KnockOut™ D-MEM/F-12 Dulbecco’s Modified Eagle Medium (D-MEM) Dulbecco’s Phosphate-Buffered Saline (D-PBS) StemPro® NSC SFM N-2 Supplement B-27® Serum-Free Supplement Neurobasal®
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