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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Histamine H1 Receptor Polyclonal Antibody_货号:E-AB-13307_多克隆抗体
- 抗体英文名:
Histamine H1 Receptor Polyclonal Antibody
- 应用范围:
WB;IHC;ELISA
- 宿主:
Rabbit
- 适应物种:
Human
- 保质期:
12个月
- 目录编号:
E-AB-13307
- 级别:
科研级别
- 库存:
999
- 供应商:
武汉伊莱瑞特生物科技股份有限公司
- 标记物:
Unconjugated
- 克隆性:
Polyclonal
- 保存条件:
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
- 形态:
液体
- 亚型:
IgG
- 规格:
200μL/120μL/60μL/20μL
| 规格: | 200μL | 产品价格: | ¥2365.0 |
|---|---|---|---|
| 规格: | 120μL | 产品价格: | ¥1640.0 |
| 规格: | 60μL | 产品价格: | ¥1010.0 |
| 规格: | 20μL | 产品价格: | ¥420.0 |
货号:E-AB-13307
| Verified Samples |
Verified Samples in WB:Human colon
Verified Samples in IHC:Human thyroid cancer,Human gastric cancer |
| Dilution |
WB 1:500-1:2000, IHC 1:50-1:200
|
| Clonality |
Polyclonal
|
| Isotype |
IgG
|
| Immunogen |
Synthetic peptide of human HRH1
|
| Abbre |
HRH1
|
| Synonyms |
BPHS;mouse;homolog of;H1 R;H1R;HH1R;HisH1;Histamine H(1) receptor;Histamine H1 receptor;Histamine receptor H1;Histamine receptor subclass H1;Hrh1;HRH1抗体
|
| Swissprot |
P35367
|
| Calculated MW |
56kDa
|
| Cellular Localization |
Cell membrane.
|
| Concentration |
1 mg/mL
|
| Storage |
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
|
| Buffer |
PBS with 0.05% sodium azide and 50% glycerol, PH7.4
|
| Purification Method |
Affinity purification
|
| Research Areas |
Immunology; Signal Transduction
|
| Conjugation |
Unconjugated
|
| Background |
Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene was thought to be intronless until recently. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily. It mediates the contraction of smooth muscles, the increase in capillary permeability due to contraction of terminal venules, the release of catecholamine from adrenal medulla, and neurotransmission in the central nervous system.
|
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Characterization of Histaminergic Receptors
Literature Cited Abstract This unit describes radioligand binding protocols for histamine H1
, and apply primary Ab only to the coverslip. Incubate 45'. Dilute antibody with Blotto. Some typical dilutions are listed below: monoclonal M1 mouse anti-Flag antibody (Sigma , Covance ) (Ca+2 sensitive) 1:350-1:500; polyclonal 12CA5 mouse anti-HA (Roche
Staining for Surface Receptors
up" on the coverslip, and the wet whatman paper helps prevent the antibody from drying up. 9. Aspirate Blotto, and apply secondary Ab. Incubate 20' in the dark. Dilute antibody with Blotto. Cy3 donkey anti-mouse (Jackson Immunoresearch ) 1:500, FITC goat anti-mouse
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