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- 文献和实验
- 技术资料
- 保存条件:
4°C, protect from light
- 库存:
货期:1-2天
- 供应商:
MedChemExpress LLC
- CAS号:
134759-22-1
- 规格:
1 mg/5 mg/10 mg
| 规格: | 1 mg | 产品价格: | ¥800.0 |
|---|---|---|---|
| 规格: | 5 mg | 产品价格: | ¥2100.0 |
| 规格: | 10 mg | 产品价格: | ¥3350.0 |
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Fluorescein Biotin
CAS No. : 134759-22-1
MCE 国际站:Fluorescein Biotin
产品活性:Fluorescein Biotin 是一种放射性生物素的替代品,它可以通过荧光或吸光度来检测和量化生物素结合位点;一经与抗生物素蛋白 (avidin) 或链霉亲和素 (streptavidin) 结合,荧光或吸光度就淬灭。
研究领域:Others
作用靶点:Fluorescent Dye
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文献和实验Labeling of Oligonucleotides with Fluorescein
Oligonucleotides are a convenient alternative to conventional, cloned DNA probes since they can be rapidly synthesized in large quantities relatively cheaply. They can be made to very specific regions of the genome, without reference
Fluorescein labeling of proteins
from free Fluorescein compounds by extensive dialysis versus PBS or by gel filtration.Concentrate with Centriprep and/or Centricon concentrators as necessary. Microfuge on high for 10 minutes and filter through 0.22 µm filter.Optionally add 0.5% azide
LABELING GC-GLOBULIN WITH FLUORESCEIN
Materials 1.2 mM PIPES,pH 7.0,500 ml. 2.Gc-globulin (Calbiochem 345802),1 mg. 3.CFSE (Carboxyfluorescein succinimidyl ester; Molecular Probes). 4.Lysine,100 mM in buffer 1,10 ml. 5.G25-150 desalting column,1 cm diameter.Equilibrated with buffer
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