DAF-FM DA,254109-22-3

Investigating G Protein-Coupled Receptor Endocytosis and Trafficking by TIR-FM

, some of which occur on a rapid timescale similar to that of functional signaling itself. Here, we describe and discuss an optical imaging approach, based on evanescent field or total internal reflection-fluorescence microscopy (TIR-FM), to investigate receptor

Studying Synaptic Transmission at the Drosophila Neuromuscular Junction Using Advanced FM 1-43 Technology

and describe two advanced protocols that make use of the membrane binding dye FM 1-43, enabling to probe deep into the mechanisms of synaptic vesicle function and dynamics at the Drosophila third instar larval neuromuscular junction.

Monitoring Rapid Endocytosis in the Electron Microscope via Photoconversion of Vesicles Fluorescently Labeled with FM1-43

of the unique fluorescent FM dyes allowed the monitoring of fast synaptic vesicle exo-endocytic cycling during live imaging sessions and after photoconversion of FM dyes into an electron-dense diaminobenzidine polymer synaptic vesicle cycling can be studied