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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
polypeptide, sealed storage, away from moisture and light, under nitrogen
- 库存:
货期:1-2天
- 供应商:
MedChemExpress LLC
- CAS号:
98849-88-8
- 规格:
1 mg/5 mg/10 mg/25 mg/50 mg/100 mg
| 规格: | 1 mg | 产品价格: | ¥500.0 |
|---|---|---|---|
| 规格: | 5 mg | 产品价格: | ¥900.0 |
| 规格: | 10 mg | 产品价格: | ¥1300.0 |
| 规格: | 25 mg | 产品价格: | ¥2390.0 |
| 规格: | 50 mg | 产品价格: | ¥3100.0 |
| 规格: | 100 mg | 产品价格: | ¥4000.0 |
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FLAG peptide
CAS No. : 98849-88-8
MCE 国际站:FLAG peptide
产品活性:FLAG peptide是一种八个氨基酸组成的多肽 (Asp-Ty-rLys-Asp-Asp-Asp-Asp-Lys),具有肠激酶切割位点。FLAG peptide用于抗体介导的重组蛋白的识别和纯化。
研究领域:Others
作用靶点:Others
In Vitro: Fusion protein technology has become an important tool for solving numerous problems linked to recombinant protein production. The properties of the additional tag facilitate identification and provide a one-step purification procedure of the fusion protein by passing cell extracts or supernatants through columns of an appropriate matrix. FLAG peptide allows elution under non-denaturing conditions. Several antibodies against FLAG peptide have been developed. One antibody, M1, binds the peptide in the presence of bivalent metal cations, preferably Ca2+. Elution is effected by chelating agents. Another strategy is competitive elution with excess of free FLAGe peptide. Antibodies M2 and M5 are applied in this procedure. The Flag-tag is first described as a calcium-dependent epitope of a monoclonal antibody. It is a highly acidic octapeptide which can be N-terminally fused to the protein of interest. As a very hydrophilic peptide the Flag–tag has a high surface probability. Flag-fusion proteins can be captured by an immunoaffinity column in the presence of Ca2+ and eluted byEDTA at low concentrations, neutral pH and thus, nearly physiological conditions.
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文献和实验Strep/FLAG Tandem Affinity Purification (SF‐TAP) to Study Protein Interactions
followed by elution with desthiobiotin. (2) Purification by the FLAG‐tag moiety: binding to anti‐FLAG M2 affinity matrix followed by elution with FLAG peptide. Abbreviations: sp., specific interactors (shown as gray circles); n.sp., nonspecific proteins
在免疫沉淀IP&免疫荧光IF如何正确的选择Flag标签抗体? Flag标签系统是已被公认的用于表达、纯化以及检测融合蛋白的表达系统,广泛应用于Western blotting、免疫细胞组化、免疫共沉淀、流式细胞术、蛋白纯化以及蛋白相互作用、蛋白分离等多个研究领域。Flag标签是一个与重组蛋白相融合的由8个亲水氨基酸(Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys)组成的多肽片断。FLAG标签只有8个氨基酸组成,因此它不会占据其他表位与结合域,避免导致融合
【共享】在免疫沉淀IP:免疫荧光IF实验中关于Flag抗体的应用
Flag标签系统是已被公认的用于表达、纯化以及检测融合蛋白的表达系统,广泛应用于Western blotting、免疫细胞组化、免疫共沉淀、流式细胞术、蛋白纯化以及蛋白相互作用、蛋白分离等多个研究领域。Flag标签是一个与重组蛋白相融合的由8个亲水氨基酸(Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys)组成的多肽片断。FLAG标签只有8个氨基酸组成,因此它不会占据其他表位与结合域,避免导致融合蛋白的功能、分泌性以及转运发生改变。由于它的亲水性特点,FLAG标签倾向于定位在融合
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