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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
Powder: -20°C, 3 years; 4°C, 2 years.In solvent: -80°C, 6 months; -20°C, 1 month.
- 库存：
货期：1-2天
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MedChemExpress LLC
- 规格：
10 mM * 1 mL///1 mg
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MMP3 inhibitor 1
CAS No. : 312930-75-9
MCE 国际站：MMP3 inhibitor 1
产品活性：MMP3 inhibitor 1 是一种有效的高选择性 MMP-3 抑制剂，IC50 为 1 nM。
研究领域：Metabolic Enzyme/Protease
作用靶点：MMP
In Vitro: MMP3 inhibitor 1 (Compound 27) inhibits MMP-1, MMP-2, MMP-9, and MMP-14 with IC50s of 14000, 529, 2420, and 20100 nM, respectively.
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文献和实验Eccles:Protein Lysates from Cells in Culture
(whether fresh lysates were made from >18month old buffer or lysates that had been stored at -80 for >18months were used). 20x Complete mini Protease Inhibitor Take 1 tablet and dissolve in 0.5mL MQH2 O by pipetting up and down. Store on ice
1. Add the following to a sterile microfuge tube on ice: -1 ug linearized plasmid DNA. -2 ul Dig RNA Labeling Mix, 10X concentrate -2 ul 10X transcription buffer -1-2 ul RNase inhibitor (DNase free) -add DEPC treated H2O up to 18 ul -2 ul RNA
RNase inhibitor (DNase free) -add DEPC treated H2O up to 18 ul -2 ul RNA polymerase as appropriate 2. Mix then spin down briefly. 3. Incubate at 37'C for 2-3 hours. 4. Add 2 ul DNase I, RNase free to remove DNA template. 5. Incubate at 37'C
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