名称:小鼠抗AU1标签抗体
货号:YT681
规格:50μl
本品为来源于小鼠的抗AU1标签的单克隆抗体
宿主:小鼠
用途:WB,ELISA,IP,ICC, IF
推荐稀释比例:WB(1:1000)
克隆性:单克隆抗体
About this Antibody
Name
AU1-tag Mouse Monoclonal Antibody
Category
Monoclonal antibody(mAb); Primary antibody
Isotype
IgG2a
Purification
ProL affinity purified
About the Immunogen
Immunogen
This antibody is produced by immunizing mice with a synthetic peptide DTYRYI (KLH-coupled).
Gene ID
-
SwissProt
-
Synonyms
-
Category
Related Products
Background
The recognized AU1 sequence represents the amino acid sequence DTYRYI, derived from the major capsid protein of bovine papillomavirus-1 (BPV-1). AU1 is a commonly used epitope tag engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized by immunochemical methods. This antibody recognizes the AU1-tag fused to the carboxyl terminus of targeted proteins.
注意事项:
1. 如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。
2. 回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
3. 提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得zuì佳的检测效果,请考虑使用上述检测专用的一抗稀释液。