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- 2025年07月11日
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文献和实验and Superdex 200p.g.) were packed into XK 16/40 or Tricorn 10/30 columns. AEC media were packed into Tricorn 10/15 columns. First experiments with ion-exchangers were conducted with HiTrap screening columns (all from GE Healthcare Bio-Sciences, Sweden). Void
, and 20 mMTris-HCl, pH 7.5, sterile filtered. 5. Syringe (30–50 mL) with 0.2-μm pore size filter. 2.3.2. Step 2: IMAC 1. Sample: Pooled Wnt3A containing fractions from Section2.3.1 . 2. HiTrap? Chelating, 1-mL column (GE Healthcare, Piscataway
at 3,000 rpm for 10 minutes at 4°C.(8) Resuspend the pellet in 1 ml 1X PBS on ice (don’t vortex or agitate) and dialyze against PBS.(9) The pharmacia HiTrap 1ml column can bind 10 micromoles of peptide or protein per ml of bed volume. Prep the HiTrap column
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