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- 详细信息
- 文献和实验
- 技术资料
- 库存:
现货
- 英文名:
EndoFree ezfilter 96-well plasmid miniprep kit
- 供应商:
上海善然生物科技有限公司
- 保存条件:
室温
- 规格:
1x96
| Buffer RET为本公司独立研发的一种可以有效吸附溶解内毒素的无色溶液。在质粒提取过程中,菌体经裂解中和,取上清后,加入该溶液,通过离心柱吸附后,既可去除内毒素。之后进行常规的洗涤、洗脱操作,即可提取到内毒素含量<0.1 EU/µg DNA的高纯度质粒,该提取方法,可在更短时间内,纯化到高纯度的质粒DNA,该质粒可直接用于转染各种内毒素敏感性细胞系,原代细胞,或者直接进行微注射。 |
| PD1818-00 | EndoFree ezfilter 96-well plasmid miniprep kit | EndoFree ezfilter 96-well plasmid miniprep kit | 1x96 | 1,350.00 |
| PD1818-01 | EndoFree ezfilter 96-well plasmid miniprep kit | EndoFree ezfilter 96-well plasmid miniprep kit | 4x96 | 5,025.00 |
| PD1818-02 | EndoFree ezfilter 96-well plasmid miniprep kit | EndoFree ezfilter 96-well plasmid miniprep kit | 20x96 | 13,115.00 |
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文献和实验Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.
Procedure for GenEluteTM Plasmid Miniprep Kit(质粒小量提取)
upon the plasmid and culture density.For best yields,follow the instructions in the note below.Transfer the appropriate volume of the recombinant E.coli culture to a microcentrifuge tube and pellet cells at ≥12000 × g for 1 minute.Discard the supernatant. Note
Miniprep/Kit-free high-throughput protocol
.--Kathleen) and yields DNA that is clean enough for sequencing or for use as a PCR template. Protocol Transfer 1.5 mL of an overnight culture containing your plasmid to an eppendorf tube and spin at 5000 rpm for 5 min in a tabletop centrifuge
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