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Rabbit anti-Goat IgG (H&L), AL

P conjugated, min. cross-reactivity to human, mouse and Rat IgG抗体
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  • ¥3244
  • 瑞典Agrisera
  • 瑞典
  • AS10 657
  • 2025年07月03日
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    • 文献和实验
    • 技术资料
    • 库存

      进口期货

    • 供应商

      上海经科化学科技有限公司

    • 抗体英文名

      Rabbit anti-Goat IgG (H&L), ALP conjugated, min. cross-reactivity to human, mouse and Rat IgG

    • 规格

      0.5mg

    Immunogen 
    Purified Goat IgG, whole molecule
    Host Rabbit
    Clonality Polyclonal
    Purity Purified rabbit IgG
    Format Liquid
    Quantity 0.5 mg
    Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
    Tested applications ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
    Recommended dilution 1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)


    名称:Rabbit anti-Goat IgG (H&L), ALP conjugated, min. cross-reactivity to human, mouse and Rat IgG
    货号:AS10 657
    品牌:Agrisera



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    图标文献和实验
    相关实验
    • 【交流】谁用过RD公司的HRP酶标羊抗鼠IgG试剂盒.

      blots. In direct ELISAs, this antibody shows less than 2% cross-reactivity with human IgG and rabbit IgG. Optimal dilutions should be determined by each laboratory for each application. 谢谢!!!

    • (共享)ELISA原理与实验方法

      , incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS

    • Double Immunohistochemistry.

      . 3- add the AP conjugated secondary antibody (50 to 100 µl) and incubate for 45 min. The secondary antibody should be absorbed against human serum; if not add 1% human serum before use.  SBA Goat anti mouse AP or Goat anti Rabbit AP can be used 1:200

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    Rabbit anti-Goat IgG (H&L), ALP conjugated, min. cross-reactivity to human, mouse and Rat IgG抗体
    ¥3244