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- 详细信息
- 文献和实验
- 技术资料
- 库存:
进口期货
- 供应商:
上海经科化学科技有限公司
- 抗体英文名:
Sheep anti-Rabbit IgG (H&L), HRP conjugated
- 规格:
1mg
| Immunogen Purified rabbit IgG (H&L) |
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Host Sheep
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Clonality Polyclonal
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Purity Affinity purified sheep IgG
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Format Lyophilized
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Quantity 1 mg
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Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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Storage Store lyophilized material at 2-8°C.
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| For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. |
| For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
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Recommended dilution This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1 : 500-1 : 5 000 (IHC), 1 : 200-1 : 5000 (WB)
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名称:Sheep anti-Rabbit IgG (H&L), HRP conjugated
货号:AS10 1538
品牌:Agrisera
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文献和实验人抗风疹病毒IgG抗体(anti-RV IgG)酶联免疫分析(ELISA)
中依次加入抗风疹病毒 IgG 抗体( anti-RV IgG ),再与 HRP 标记的抗原结合,形成抗原 - 抗体 - 酶标抗原复合物,经过彻底洗涤后加底物 TMB 显色。 TMB 在 HRP 酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的抗风疹病毒 IgG 抗体( anti-RV IgG )呈正相关。用酶标仪在 450nm 波长下测定吸光度( OD 值),通过标准曲线计算样品中人抗风疹病毒 IgG 抗体( anti-RV IgG )浓度。 试剂盒组成
E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
酶与抗体交联的方法有许多种 , 根据酶的结构不同可采用不同的方法。对于制备 HRP 结合物,可用戊二醛二步法和过碘酸钠法。尤以简易过碘酸钠法更为常用。 戊二醛二步法 1. 原理:戊二醛为一种双功能试剂,通过其醛基分别与酶和免疫球蛋白上的氨基共价结合,形成酶 - 戊二醛 - 免疫球蛋白结合物。 2. 标记步骤: (1) 称取 HRP25
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