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- 详细信息
- 文献和实验
- 技术资料
- 库存:
进口期货
- 供应商:
上海经科化学科技有限公司
- 抗体英文名:
Rabbit anti-Chicken IgY (H&L), TRITC conjugated
- 规格:
1mg
| Immunogen Purified Chicken IgG/Y (H&L) |
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Host Rabbit
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Clonality Polyclonal
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Purity Affinity purified rabbit IgG
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Format Lyophilized
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Quantity 1 mg
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Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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Storage Store lyophilized material at 2-8°C.
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| For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. |
| For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
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Recommended dilution The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications.
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名称:Rabbit anti-Chicken IgY (H&L), TRITC conjugated
货号:AS10 1490
品牌:Agrisera
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Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
serum, obtained from the same species as the secondary antibody. Depending on the method employed, use anti-rabbit IgG (Fc) horseradish peroxidase (HRP) conjugate (1:100 dilution), a biotin-conjugated secondary antibody, or a fluorescence-conjugated
Double immunofluorescence staining for BCL-6 and else.
. Example: Pirmary antibodies: rabbit anti-BCL-6 0.5µg/ml in PBS/BSA/Azide + mouse anti HA supn 1:100. Secondary antibodies: biotin-conjugated goat anti rabbit (1:200) + TRITC conjugated goat anti-mouse (1:200). Alternatively, biotin-conjugated
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