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- 详细信息
- 文献和实验
- 技术资料
- 库存:
进口期货
- 供应商:
上海经科化学科技有限公司
- 抗体英文名:
Goat anti-biotin, HRP conjugated
- 规格:
1mg
| Immunogen Biotin |
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Host Goat
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Clonality Polyclonal
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Purity Affinity purified IgG
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Format Lyophilized
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Quantity 1 mg
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Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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Storage Store lyophilized material at 2-8°C.
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| For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. |
| For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
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Recommended dilution This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator.
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名称:Goat anti-biotin, HRP conjugated
货号:AS10 1454
品牌:Agrisera
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文献和实验一、戊二醛二步法1. 原理戊二醛为一种双功能试剂,通过其醛基分别与酶和免疫球蛋白上的氨基共价结合,形成酶-戊二醛-免疫球蛋白结合物。2. 标记步骤(1)称取HRP25mg溶于1.25%戊二醛溶液中,于室温静置过夜。(2)反应后的酶溶液经Sephadex G-25层析柱,用生理盐水洗脱。流速控制在1ml/1分钟,收集棕色流出液。如体积大于5ml,则以PEG浓缩至5ml。放置25ml小烧杯中,缓慢搅拌。(3)将待标记的抗体12.5mg用生理盐水稀释至5ml,搅拌下逐滴加入酶溶液中。(4)用1M
A variety of staining reactions for the visualization of cellular and extracellular glycoconjugates at the light microscopic level are available that are based on the detection of carboxyl and sulfate groups or periodic acid reactive
酶与抗体交联的方法有许多种,根据酶的结构不同可采用不同的方法。对于制备HRP结合物,可用戊二醛二步法和过碘酸钠法。尤以简易过碘酸钠法更为常用。 戊二醛二步法 1. 原理:戊二醛为一种双功能试剂,通过其醛基分别与酶和免疫球蛋白上的氨基共价结合,形成酶-戊二醛-免疫球蛋白结合物。 2. 标记步骤: (1) 称取HRP25mg溶于1.25%戊二醛溶液中,于室温静置过夜。 (2) 反应后的酶溶液经Sephadex G-25层析柱,用生理盐水洗脱。流速控制
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