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- 详细信息
- 文献和实验
- 技术资料
- 库存:
进口期货
- 供应商:
上海经科化学科技有限公司
- 抗体英文名:
Goat anti-Mouse IgG (H&L), FITC conjugated, min. cross-reactivity to bovine,human, rabbit, rat IgG
- 规格:
1mg
| Host Goat |
|
Clonality Polyclonal
|
|
Purity Affinity purified IgG
|
|
Format Liquid
|
|
Quantity 1 mg
|
|
Storage Store at 2-8°C. Shelf life of this product is one year from date of receipt.
|
|
Recommended dilution 1 : 20-1 : 2000 (IHC), 1 : 50-1 : 5000 (ICC) (Flow cyt)
|
名称:Goat anti-Mouse IgG (H&L), FITC conjugated, min. cross-reactivity to bovine,human, rabbit, rat IgG
货号:AS10 1156
品牌:Agrisera
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文献和实验Immunohistochemistry using Anti-Ganglioside Antibodies
supernatant containing antiganglioside mAb overnight at 4°C. The time of incubation may range from 30 min. to overnight as determined by experience. 5. Wash with 1% BSA in PBS 4 times. 6. Incubate in FITC-conjugated goat anti-mouse IgM F(ab
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
Dual- and Triple-Color Fluorospot
, in PBS containing 0.1% bovine serum albumin (BSA) (seeNote 8). Add 100 μl/well and leave the plate for 2 h at room temperature. c. Wash the plate as in step 6 above. d. Dilute secondary detection reagents, i.e., mAb anti-FITC
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