- ￥3441
- 瑞典Agrisera
- 瑞典
- AS10 1106
- 2025年07月08日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
进口期货
- 供应商：
上海经科化学科技有限公司
- 抗体英文名：
Rabbit anti-Goat IgG Fc, HRP conjugated, min. cross-reactivity to human serum
- 规格：
1mg
| Host Rabbit |
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Clonality Polyclonal
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Purity Affinity purified IgG
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Format Lyophilized
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Quantity 1 mg
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Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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Storage Store lyophilized material at 2-8°C.
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| For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. |
| For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
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Recommended dilution 1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB)
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名称：Rabbit anti-Goat IgG Fc, HRP conjugated, min. cross-reactivity to human serum
货号：AS10 1106
品牌：Agrisera
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文献和实验blots. In direct ELISAs, this antibody shows less than 2% cross-reactivity with human IgG and rabbit IgG. Optimal dilutions should be determined by each laboratory for each application. 谢谢!!!
ELISA Procedure for Measuring Serum Antibody Titer
Phosphatase Reagents for Alkaline Phosphatase DetectionGoat anti-species IgG whole molecule/alkaline phosphatase conjugate that is appropriate for the source of your antiserum. For example, if the antibodies were raised in a rabbit, use goat anti-rabbit IgG
. 3- add the AP conjugated secondary antibody (50 to 100 µl) and incubate for 45 min. The secondary antibody should be absorbed against human serum; if not add 1% human serum before use. SBA Goat anti mouse AP or Goat anti Rabbit AP can be used 1:200
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