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- 详细信息
- 文献和实验
- 技术资料
- 库存:
进口期货
- 供应商:
上海经科化学科技有限公司
- 抗体英文名:
Rabbit anti-Goat IgG (H&L), F(ab)'2 fragment, HRP conjugated, min. cross-reactivity to human, mouse,Rat IgG
- 规格:
0.5mg
| Host Rabbit |
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Clonality Polyclonal
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Purity Affinity purified IgG, F(ab)'2 fragment
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Format Lyophilized
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Quantity 0.5 mg
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Reconstitution For reconstitution add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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Storage Store lyophilized material at 2-8°C.
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| For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. |
| For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
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Recommended dilution The optimal working dilution should be determined by the investigator.
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名称:Rabbit anti-Goat IgG (H&L), F(ab)'2 fragment, HRP conjugated, min. cross-reactivity to human, mouse,Rat IgG
货号:AS10 1065
品牌:Agrisera
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文献和实验E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
. Incubate 1 hr @ RT with shaking. Wash plate 3x and add 100 µl/well anti-rabbit HRP conjugated antibody diluted 1:1000 in assay buffer. Incubate 1 hr @ RT w/shaking. Wash 3x and add 100 µl/well TMB. Allow to develop w/o shaking
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
serum, obtained from the same species as the secondary antibody. Depending on the method employed, use anti-rabbit IgG (Fc) horseradish peroxidase (HRP) conjugate (1:100 dilution), a biotin-conjugated secondary antibody, or a fluorescence-conjugated
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