- ￥3441
- 瑞典Agrisera
- 瑞典
- AS10 1045
- 2025年07月08日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
进口期货
- 供应商：
上海经科化学科技有限公司
- 抗体英文名：
Rabbit anti-Goat IgG (H&L), F(ab)'2 fragment, HRP conjugated
- 规格：
0.5mg
| Host Rabbit |
|
Clonality Polyclonal
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Purity Affinity purified IgG, F(ab)'2 fragment
|
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Format Lyophilized
|
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Quantity 0.5 mg
|
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Reconstitution For reconstitution add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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Storage Store lyophilized material at 2-8°C.
|
| For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. |
| For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
|
Tested applications ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
|
|
Recommended dilution 1 : 500-1 : 5000 (IHC),1 : 200 -1 : 5 000 (WB)
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名称：Rabbit anti-Goat IgG (H&L), F(ab)'2 fragment, HRP conjugated
货号：AS10 1045
品牌：Agrisera
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文献和实验E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
. Incubate 1 hr @ RT with shaking. Wash plate 3x and add 100 µl/well anti-rabbit HRP conjugated antibody diluted 1:1000 in assay buffer. Incubate 1 hr @ RT w/shaking. Wash 3x and add 100 µl/well TMB. Allow to develop w/o shaking
Anti-GFP/BrdU In Situ Immunostaining
IgG (Jackson ImmunoResearch, 111-096-006) Biotinylated anti-BrdU (AbCam, ab2284-125) Streptavidin-Alexa Flour 555 (Invitrogen/Molecular Probes, S21381) Goat serum Horse serum BSA 2N HCl DAPI Fluorescent mounting medium (Vectashield
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