Donkey anti-Rabbit IgG (H&L),
HRP conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, sheep IgG抗体- ￥3244
- 瑞典Agrisera
- 瑞典
- AS10 1008
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
进口期货
- 供应商：
上海经科化学科技有限公司
- 抗体英文名：
Donkey anti-Rabbit IgG (H&L), HRP conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, sheep IgG
- 规格：
1mg
| Host Donkey |
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Clonality Polyclonal
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Purity Affinity purified IgG
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Format Lyophilized
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Quantity 1 mg
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Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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Storage Store lyophilized material at 2-8°C.
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| For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. |
| For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
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Recommended dilution The optimal working dilution should be determined by the investigator.
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名称：Donkey anti-Rabbit IgG (H&L), HRP conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, sheep IgG
货号：AS10 1008
品牌：Agrisera
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
serum, obtained from the same species as the secondary antibody. Depending on the method employed, use anti-rabbit IgG (Fc) horseradish peroxidase (HRP) conjugate (1:100 dilution), a biotin-conjugated secondary antibody, or a fluorescence-conjugated
, 5% BSA, pH 7.4) for 2 h at room temperature and then probed for CTGF by incubation for 40 min with anti-CTGF antibody at 0.5 µg/ml in blocking buffer. After extensive washing at 37°C, the filters were incubated with either HRP-conjugated donkey anti
. 3- add the AP conjugated secondary antibody (50 to 100 µl) and incubate for 45 min. The secondary antibody should be absorbed against human serum; if not add 1% human serum before use. SBA Goat anti mouse AP or Goat anti Rabbit AP can be used 1:200
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