Goat anti-Rat IgG (H&L), HRP c
onjugated, min. cross-reactivity to human, mouse lgG (highly absorbed against mouse IgG)抗体- ￥2880
- 瑞典Agrisera
- 瑞典
- AS09 618
- 2025年07月08日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
进口期货
- 供应商：
上海经科化学科技有限公司
- 抗体英文名：
Goat anti-Rat IgG (H&L), HRP conjugated, min. cross-reactivity to human, mouse lgG (highly absorbed against mouse IgG)
- 规格：
1mg
| Immunogen |
| Purified Rat IgG, whole molecule |
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Host Goat
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Clonality Polyclonal
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Purity Purified goat IgG
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Format Lyophilized
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Quantity 1 mg
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Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilutions daily.
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Storage Store non-diluted antibody at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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Tested applications ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
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Recommended dilution 1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 10 000 -1 : 50 000 (WB)
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名称：Goat anti-Rat IgG (H&L), HRP conjugated, min. cross-reactivity to human, mouse lgG (highly absorbed against mouse IgG)
货号：AS09 618
品牌：Agrisera
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文献和实验blots. In direct ELISAs, this antibody shows less than 2% cross-reactivity with human IgG and rabbit IgG. Optimal dilutions should be determined by each laboratory for each application. 谢谢!!!
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
Immunohistochemistry using Anti-Ganglioside Antibodies
supernatant containing antiganglioside mAb overnight at 4°C. The time of incubation may range from 30 min. to overnight as determined by experience. 5. Wash with 1% BSA in PBS 4 times. 6. Incubate in FITC-conjugated goat anti-mouse IgM F(ab
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