- ￥2880
- 瑞典Agrisera
- 瑞典
- AS09 604
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
进口期货
- 供应商：
上海经科化学科技有限公司
- 抗体英文名：
Donkey anti-Sheep IgG (H&L), HRP conjugated
- 规格：
1mg
| Immunogen |
| Purified sheep IgG, whole molecule |
|
Host Donkey
|
|
Clonality Polyclonal
|
|
Purity Purified donkey IgG
|
|
Format Lyophilized
|
|
Quantity 1 mg
|
|
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
|
|
Storage Store lyophilized material at 2-8°C. For storage at -20°C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
|
|
Tested applications ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
|
|
Recommended dilution 1 : 200 -1 : 5 000 (ELISA), 1 : 200-1 : 5 000 (WB) 1 : 500 -1 : 5000 (IHC)
|
名称：Donkey anti-Sheep IgG (H&L), HRP conjugated
货号：AS09 604
品牌：Agrisera
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文献和实验人抗风疹病毒IgG抗体（anti-RV IgG）酶联免疫分析（ELISA）
中依次加入抗风疹病毒 IgG 抗体（ anti-RV IgG ），再与 HRP 标记的抗原结合，形成抗原 - 抗体 - 酶标抗原复合物，经过彻底洗涤后加底物 TMB 显色。 TMB 在 HRP 酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的抗风疹病毒 IgG 抗体（ anti-RV IgG ）呈正相关。用酶标仪在 450nm 波长下测定吸光度（ OD 值），通过标准曲线计算样品中人抗风疹病毒 IgG 抗体（ anti-RV IgG ）浓度。 试剂盒组成
E L I S A w i t h p l a t e l e t s
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我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
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