- ￥4305
- 瑞典Agrisera
- 瑞典
- AS15 2860
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
进口期货
- 供应商：
上海经科化学科技有限公司
- 抗体英文名：
Streptavidin HRP Conjugated, for ELISA
- 规格：
1mg
| Quantity 1 mg |
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Storage Store freeze-dried (lyophilized) powder at Store at 2-8°C. Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily.Shelf life of this product is 1 year from date of receipt.
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Tested applications This conjugate is designed to be used in the detection of biotinylated primary antibodies. It is suitable for use in ELISA (ELISA), WB, IHC.
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Recommended dilution The optimal working dilution should be determined by the investigator.Suggested starting dilution(s):1 : 500-1 : 2000 1 : 50 000-1 : 5 000 (WB)
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名称：Streptavidin HRP Conjugated, for ELISA
货号：AS15 2860
品牌：Agrisera
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文献和实验for 6 months without reducing binding activity. 2) This buffer can not be used for diluting HRP conjugated antibodies since sodium azide is an inhibitor of HRP. HRP-conjugated Primary Antibody Dilution Buffer: 1%BSA
once and for 5 minutes twice.4. Secondary antibody incubation: Incubate the blot in 1 % non-fat milk / PBST or Trident Universal Protein Blocking Reagent containing the HRP-conjugated secondary antibody at the proper dilution for one hour at RT.5. Washing: Wash the blot
我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
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