- ¥500
- BOSSBIO
- M-0004-B
- USA
- 2025年11月25日
- HC-P IHC WB ELISA IC
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
BOSSBIO
- 应用范围：
HC-P IHC WB ELISA IC
- 宿主：
Mouse
- 供应商：
博胜经纬
- 级别：
免疫球蛋白
- 库存：
大量
- 抗原来源：
Mouse
- 保质期：
一年以上
- 是否单克隆：
多克隆
- 保存条件：
-20℃
- 规格：
10mg
Mouse IgG （ Immunoglobulines Antigen )
Quantity size: 10 mg packaging: Lyophilized form
Host species： Mouse
Product type : Immunoglobulines Antigen - Mouse IgG
Cross reactivity: Reacts with Mouse IgG
Purification Method: Purified by Protein-A affinity chromatography..purity :>95%
Tested applications: · Use for biotechnology
Dilution method : pH 7.2 0.01 M PBS or Sterile water dilution
Please Note: Optimal dilutions should be determined by each laboratory for each application.
Storage instructions: Store at 2-4 ºC short term ( 4-6 Month ). Aliquot and store at -20ºC long term( 1-2 Years). Avoid repeated freeze / thaw cycles.
Background:
Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Company Name :Beijing Bossbio Bio-Technology Co.,Ltd
Address: Room 910,Building 6,Hefeng season ,Chengzhuang South, Chengzhuang Road, High-Tech Park, Fengtai District,Beijing ,China 100071
Product Order Email :
1.bossbio@163.com
2.antibodies @bossbio.com (Antigens, antibodies, Elisa kits biology
Technical Sevice Email : support@bossbio.com
After-sales Service Email : after-sales @bossbio.com
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
buffer (pH9.0) ~ 200 ml. Filter protein mixture Add protein mixture or culture supernatant containing Ab (adjust pH to 7.8 with Binding buffer ; red color) to the Protein A column. Mouse antibodies of the IgG1 subclass
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
. A cheap alternative is to exploit the relative lower levels of each IgG isotype in serum and on follicular B cells to stain with isotype-specific, conjugated, anti-mouse secondary antibodies. IgG isotypes account each for 20-25% ot total serum
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