线粒体膜电位分析试剂盒（JC-1法）

线粒体膜电位分析试剂盒（JC-1法） - KTA4001

核心产品｜细胞分析｜细胞凋亡/周期检测

• 应用领域：细胞健康状态评估，细胞凋亡/周期早期检测
• 检测原理：JC-1荧光探针通过红绿荧光转变反映线粒体膜电位变化
• 产品形态：即用型冻干试剂，含特异性氧化磷酸化解偶联剂

核心优势与技术特性

• ► 双色荧光可视化：
  健康线粒体显示红色聚集态荧光，膜电位下降时转为绿色单体荧光
• ► 专业检测系统：
  经流式细胞仪（Ex/Em=585/590nm）与荧光显微镜（Ex/Em=514/529nm）双重验证
• ► 标准化对照体系：
  配备50mM CCCP作为阳性对照，确保实验有效性验证

产品规格与订购信息

实验操作关键点

• • 染料预处理：试剂盒含100×JC-1浓缩液，需按比例稀释后避光使用
• • 光敏感操作：全程避光孵育，建议使用铝箔包裹培养器皿
• • 结果判读窗口：染色后1小时内完成荧光信号采集

运输存储：干冰运输，组分保存条件详见产品说明
质量声明：批次QC报告包含CCCP效价验证及荧光本底检测数据

重要注意事项

• ▲ 试剂复温：使用前需37℃平衡30分钟，避免结晶析出
• ▲ 设备兼容性：推荐使用配备488nm激光器的流式细胞仪
• ▲ 应用限制：不可用于临床诊断，神经细胞系需额外验证

免责声明：本产品受专利法约束，使用者需自行确认实验合规性，制造商不承担因使用方式引发的知识产权风险。 ```

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mitochondrial Membrane Potential Assay Kit (JC-1 Method) - KTA4001

Core Product｜Cell Analysis｜Apoptosis/Cell Cycle Detection

• Applications: Cell health status assessment, early-stage apoptosis/cell cycle detection
• Detection Principle: JC-1 fluorescent probe reflects mitochondrial membrane potential changes through red-green fluorescence shift
• Product Form: Ready-to-use lyophilized reagent containing specific oxidative phosphorylation uncoupler

Core Advantages & Technical Features

• ► Dual-color fluorescence visualization:
  Healthy mitochondria exhibit red J-aggregate fluorescence, shifting to green monomeric fluorescence upon membrane potential dissipation
• ► Professional detection system:
  Dually validated by flow cytometry (Ex/Em=585/590nm) and fluorescence microscopy (Ex/Em=514/529nm)
• ► Standardized control system:
  Includes 50mM CCCP as positive control for experimental validity verification

Specifications & Ordering Information

Key Experimental Procedures

• • Dye pretreatment: Kit contains 100× JC-1 concentrate, requires proportional dilution and light-protected usage
• • Light-sensitive operation: Maintain light-protected incubation throughout, recommend aluminum foil-wrapped culture vessels
• • Result interpretation window: Complete fluorescence signal acquisition within 1 hour post-staining

Shipping & Storage: Dry ice shipment, component storage conditions detailed in product manual
Quality Statement: Batch QC reports include CCCP potency validation and fluorescence background detection data

Important Notes

• ▲ Reagent equilibration: Requires 37℃ balancing for 30 minutes before use to prevent crystallization
• ▲ Equipment compatibility: Recommended for flow cytometers equipped with 488nm laser
• ▲ Application limitations: Not for clinical diagnosis, neural cell lines require additional validation

Disclaimer: This product is protected by patent law. Users must independently confirm experimental compliance. The manufacturer assumes no liability for intellectual property risks arising from usage methods. ```

Note: The above information may be subject to changes due to product optimization and upgrades. For specific details, please consult Yakoyin Company.