英文名称 Anti-AuroraA/ARK-1/AURKA抗体 中文名称 有丝分裂激酶A抗体 别 名 AIK; ARK1; AURA; AURKA; Aurora 2; Aurora A; Aurora Family Kinase 1; aurora kinase A; Aurora Related Kinase 1; Aurora/IPL1 Like Kinase; Aurora/IPL1 Related Kinase 1; AURORA2; AYK1; BRAK; Breast Tumor Amplified Kinase; BTAK; hARK1; IAK; IPL1 Aurora Related Kinase 1; IPL1 Related Kinase; MGC34538. 浓 度 1mg/1ml 规 格 0.1ml/100μg 0.2ml/200μg 抗体来源 Rabbit 克隆类型 polyclonal 交叉反应 Human, Mouse, Rat, Pig, Cow, Horse, Rabbit 产品类型 一抗 研究领域 肿瘤 细胞生物 细胞周期蛋白 激酶和磷酸酶 蛋白分子量 predicted molecular weight: 48kDa 性 状 Lyophilized or Liquid 免 疫 原 KLH conjugated synthetic peptide derived from human Auroa A C-terminus 产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 亚 型 IgG 纯化方法 affinity purified by Protein A 储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide 产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. 保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 抗体的生物素化标记实验要点: 1.Anti-AuroraA/ARK-1/AURKA抗体如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析; 2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件; 3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活; 4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等; 5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性; 6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合; 7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用; 8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。 无水三氯铝 1Butyl3methylimidazolium Bromide 3,4,7,8四1,10菲罗啉 1Butyl3methylimidazolium chloride,... 四亚二磷脂 1Methyl3propylimidazolium iodide 35 EMIMPF6 2酰 1Ethyl3methylimidazolium bromide, ... 1,2双(二膦) Tetrabutylammonium hydroxide 30hydrat... 丙二酰 EMIMBF4 3(4)丙 (5Carboxypentyl)triphenylphosphonium ... 3(3)丙 Bis[tetrakis(hydroxymethyl)phosphonium... 联二氯苄 Tetrabutylphosphonium bromide ,≥98.0% 特价促销 5噻酚2腈 Acephate 42 Phosphorus(V) oxybromide 丙二 Phosphorus pentabromide 吲哚5羧 Phosphorus pentasulfide 2羟6萘 (Methoxymethyl)triphenylphosphonium ch...