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深圳子科生物科技有限公司
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相关产品:
| PCR试剂 | G008 | Taq DNA Polymerase | 5000u |
| G126 | Taq DNA Polymerase | 10000U | |
| G009 | Taq DNA Polymerase | 1000U(200 μl) | |
| G013 | 2X PCR Taq MasterMix | 5.0 ml (200 Rxns) | |
| G013-dye | 2X PCR Taq MasterMix with dye | 5.0 ml (200 Rxns) | |
| G472 | Safe-Green ; 2X PCR Taq MasterMix | 5.0 ml (200 Rxns) | |
| G012 | Taq Plus DNA Polymerase | 250 U (50 µl) | |
| G040 | Taq Plus DNA Polymerase | 1000U(200 µl) | |
| G014 | 2X PCR Taq Plus MasterMix | 5.0 ml (200 Rxns) | |
| G014-dye | 2X PCR Taq Plus MasterMix with dye | 5.0 ml (200 Rxns) | |
| G277 | TaqFast DNA Polymerase | 250 U (50 µl) | |
| G278 | TaqFast DNA Polymerase | 1000U(200 μl) | |
| G280 | 2X PCR TaqFast MasterMix | ||
| G280-dye | 2X PCR TaqFast MasterMix with dye | ||
| G078 | Precision ; DNA Polymerase | 500 U (100 µl) | |
| G011 | HotStart DNA Polymerase | 250 U (50 µl) | |
| G039 | HotStart DNA Polymerase | 1000U(200 µl) | |
| G010 | dNTP Mix | 250µl | |
| G128 | dNTP Mix | 500 µl | |
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文献和实验We report a simple homogeneous fluorescence assay for quantification of DNA polymerase function in high throughput. The fluorescence signal is generated by the DNA polymerase triggering opening of a molecular beacon extension of the template
The Polymerase Chain Reaction (PCR)
. Did He Really Invent PCR? • The basic principle of replicating a piece of DNA using two primers had already been described by Gobind Khorana in 1971:– Kleppe et al. (1971) J. Mol. Biol. 56, 341-346. • Progress was limited by primer synthesis and polymerase
能催化从四种5′-脱氧核苷三磷酸游离出焦磷酸而使DNA进行聚合反应的酶。EC2.7.7.7.。必须以DNA为模板,合成的DNA与模板具有互补的碱基排列(碱基互补性)。是与DNA复制和修复有关的重要的酶,虽可从许多组织分离出来,但对大肠杆菌的这种酶的研究较为先进。康伯格(A.Kornberg,1958)最初从大肠杆菌将此酶纯化,并弄清了其结构和特异性,但以后经过分子遗传学的研究,已明确了这种酶主要与DNA修复有关。因此重新进行与DNA复制有关酶的检索,发现两种新的酶。康伯格最初发现的酶称为
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