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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 保质期:
12 months from the shipping date of the product.
- 英文名:
Human IDH1 protein, His tag (active)
- 库存:
Available
- 供应商:
GeneTex
- 规格:
100 μg
3 μg of GTX67054-pro Human IDH1 protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain
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文献和实验Generation of Monoclonal Antibodies to Native Active Human Glycosyltransferases
Complex carbohydrates serve a wide range of biological functions in cells and tissues. Their biosynthesis involves more than 200 distinct glycosyltransferases in human cells, and the expression, properties, and topology of these enzymes
附加FLAG tag、PA tag、IDH1 (IDH1-FLAG-PA)的骨肉瘤细胞裂解液为电泳样品。(A)泳道1在一次抗体中加入1μg/Mlnz-1,二抗使用抗大鼠Pa tag NZ-1抗体,泳道2在一抗中加入3.5μg/Mlm2,二抗使用抗小鼠FLAG抗体,这两个条件的样品的转录膜经CBB染色再进行比较。结果由于二抗不同产生了一些差别,用PA TAG/NZ-1法可以很好地见到目的条带。(B)在一抗中加入1μg/Mlnz-1,二抗使用抗大鼠Pa tag NZ-1抗体的条件下,对一次抗体
Purification of the human TATA-Binding Protein, TBP
The TATA-binding protein TBP plays a central role in eukaryotic transcription initiation (1 , 2 ). TBP binds to the promoter consensus sequence TATAAA, located approx 30 nucleotides upstream of the transcriptional start site. Once bound, TBP
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