- ￥6200
- GeneTex
- 美国
- GTX65640-pro
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
- 保质期：
12 months from the shipping date of the product.
- 英文名：
Human LAG3 protein, human IgG1 Fc tag (active)
- 库存：
Available
- 供应商：
GeneTex
- 规格：
50 μg
LAG3 human Fc fusion protein (GTX65640-pro) induced dendritic cells (DC) maturation. Method: Immature DCs derived from human monocytes, resuspended at 1x106 cells/ml in complete culture medium, are incubated with LAG3 human Fc fusion protein (GTX65640)(10μg/ml), LPS (positive control, 5μg/ml) or human IgG1 (isotype-matched negative control, 10μg/ml) for 48 hours. DC maturation induced by LAG3 human Fc fusion protein or LPS is assessed by the increased expression of MHC Class II, CD80, CD83, CD86 and CD40.
Binding of LAG3 human Fc fusion protein (GTX65640-pro) on dendritic and B cells. Method: Monocyte-derived immature DC (iDC), DC matured for 48h with LPS (mDC) and EBV-transformed B-cells are incubated with either an anti-MHC class II MAb (10μg/ml) or GTX65640 LAG3 human Fc fusion protein (10μg/ml) for 30 min. at 4ºC in PBS/1% BSA. The cells are then stained for 30 min. at 4ºC with anti-human Fc antibody.
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