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背景介绍
巨细胞病毒(CMV)感染是器官移植术后一种常见的并发症，也是术后早期最主要的感染和死亡原因。据报道，CMV活动性感染率在肾移植受者中为50-75%，其中有10-30%的患者发展为有症状的CMV活动性感染。人外周血CMV pp65的检测可以帮助诊断急性或复发性CMV感染，荷兰IQ 公司的CMV Brite Turbo Kit能在EDTA或肝素化的抗凝人外周血中，以免疫荧光的方法快速检测CMV pp65,在器官移植病人中得到了广泛的应用。
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文献和实验DNA Extraction Promega DNA-IQ System Database Protocol
or pair of tweezers Container of sterile 1.5 mL microcentrifuge tubes DNA-IQ magnetic stand Biohazard waste container Set of micropipettors One small beaker for waste Calculator Timer Ultra-fine point sharpie
Natural Products or Not How to Screen for Natural Products in the Emerging HTS Paradigm
Natural products are undeniably the best source for diversity in chemotype for the discovery of novel therapeutics. One-third of the top selling drugs in the world are natural products or their derivatives (Fig. 1 ). Roughly 60
It is often necessary to obtain DNA sequence information following successful amplification. Although polymerase chain reaction (PCR) products may be cloned into a vector and sequenced at a later stage, it is often preferable to sequence PCR
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