Kainos

The FGF-23 ELISA Kit is a two-site enzyme-linked immunosorbent assay kit for the measurement of FGF-23 in serum. Two specific murine monoclonal antibodies bind to full-length FGF-23. One antibody is immobilized onto the microtiter plate well for capture. The other antibody is conjugated to HRP(horseradish peroxidase) for detection.

In first reaction, a sample containing FGF-23 is incubated with the immobilized antibody in a microtiter well. FGF-23 in the sample is captured with the antibody. At the end of this reaction, the well is washed to remove unbound FGF-23 and other components.

In second reaction, this immobilized FGF-23 is incubated with HRP labeled antibody to form a “sandwich”complex :

Anti FGF-23 antibody - N-terminal　FGF-23　C-terminal - HRP labeled anti FGF-23 antibody