The bladder is a hollow sphere, with the wall of the sphere comprising the serosa, muscularis, submucosa, musculris mucosa, and lamina propria [1]. Embedded within these structures is a circulatory system, sensory and motor neurons, and an immune system. The endothelial cells lining the microvasculature of the circulatory system are known to play a critical “gatekeeper” role in the inflammatory process through their ability to recruit circulating immune cells into tissues and foci of inflammation. Stimulation of endothelial cells in the bladder wall by thrombin or tryptase may result in a similar patter of phospholipid metabolite production and may play a role in the inflammatory process in IC [2]. To be able to study the role of endothelial cell in physiological and inflammatory condition, the HBdMEC culture provides a useful in vitro model.
HBdMEC from ScienCell Research Laboratories are isolated from human bladder tissue. HBdMEC are cryopreserved at the end of secondary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HBdMEC are characterized by immunofluorescent method with antibodiesto VWF/Factor VIII and CD31 (P-CAM) and by uptake of DiI-Ac-LDL. HBdMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBdMEC are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.
Recommended Medium It is recommended to use Endothelial Cell Medium (ECM, Cat. No. 1001) for the culturing of
HBdMEC.
Product Use
HBdMEC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping
Dry ice.
Reference [1] Hossler FE and Monson FC. Microvasculature of the rat urinary bladder. Anat Rec
243: 438?448, 1995. [2]. Alice Rickard, Craig Portell, Pamela J. Kell, Suzanne M. Vinson and Jane McHowat (2005 ) Protease-activated receptor stimulation activates a Ca 2+-independent phospholipase A2 in bladder microvascular endothelial cells. Am J Physiol Renal Physiol 288:F714-F721.