- ￥1350 - 3000
- Proteintech
- 66323-1-Ig
- 2025年10月13日
- WB, IHC, IF, ELISA
- Mouse
- human, pig, zebrafish
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human Glutamine synthetase protein
- 亚型：
IgG2b
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human, pig, zebrafish
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Mouse
- 应用范围：
WB, IHC, IF, ELISA
- 靶点：
Glutamine Synthetase
- 抗体英文名：
Glutamine Synthetase Monoclonal antibody
- 抗体名：
Glutamine Synthetase Monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | Jurkat cells, pig liver tissue, pig brain tissue |
| Positive IHC detected in | human liver cancer tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | zebrafish retina, human brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66323-1-Ig targets Glutamine Synthetase in WB, IHC, IF, ELISA applications and shows reactivity with human, pig, zebrafish samples.
| 经测试应用 | WB, IHC, IF, ELISA |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, pig, zebrafish |
| 文献引用反应性 | human, mouse, rat, zebrafish |
| 免疫原 |
CatNo: Ag6309 Product name: Recombinant human Glutamine synthetase protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-373 aa of BC011700 Sequence: MTTSASSHLNKGIKQVYMSLPQGEKVQAMYIWIDGTGEGLRCKTRTLDSEPKCVEELPEWNFDGSSTLQSEGSNSDMYLVPAAMFRDPFRKDPNKLVLCEVFKYNRRPAETNLRHTCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGPYYCGVGADRAYGRDIVEAHYRACLYAGVKIAGTNAEVMPAQWEFQIGPCEGISMGDHLWVARFILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKYIEEAIEKLSKRHQYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRSASIRIPRTVGQEKKGYFEDRRPSANCDPFSVTEALIRTCLLNETGDEPFQYKN |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | glutamate-ammonia ligase (glutamine synthetase) |
| 别名 | GLNS, GLUL, Glutamate ammonia ligase, Glutamate decarboxylase, Glutamine synthetase, GS, PIG43, PIG59 |
| 计算分子量 | 374 aa, 42 kDa |
| 观测分子量 | 42 kDa |
| GenBank蛋白编号 | BC011700 |
| 基因名称 | Glutamine Synthetase |
| Gene ID (NCBI) | 2752 |
| RRID | AB_2881703 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P15104 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts (PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). There are other bands with higher (66 kDa, 97 kDa) and lower (30 kDa)molecular weights also detected besides the 42 kDa band indicating the proteolysis of GLUL protein by the ubiquitin system(PMID:10091759).
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文献和实验| Species | Application | Title |
|---|---|---|
J Hepatol OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.Authors - Weiqi Dai | ||
| mouse | IF | Acta Neuropathol Selective vulnerability of tripartite synapses in amyotrophic lateral sclerosis.Authors - Matthew J Broadhead |
J Neurosci C9ORF72 deficiency results in neurodegeneration in the zebrafish retinaAuthors - Natalia Jaroszynska | ||
| zebrafish | IF | Life Sci Alliance Unique activities of two overlapping PAX6 retinal enhancersAuthors - Kirsty Uttley |
| rat | IF | In Vitro Cell Dev Biol Anim TRIM9 promotes Müller cell-derived retinal stem cells to differentiate into retinal ganglion cells by regulating Atoh7Authors - Qi Zeng |
| mouse | IF | PLoS One Excitatory to inhibitory synaptic ratios are unchanged at presymptomatic stages in multiple models of ALSAuthors - Calum Bonthron |
缩写为 GS。为在 ATP的存在下使氨与谷氨酸结合生成谷氨酰胺的酶， EC． 6． 3． 1． 2． L-谷氨酸 氨 ATPL- 谷氨酰胺 ADP Pi反应机制很复杂，要经过酶蛋白与γ -谷氨酰磷酸的中间体。与谷氨酰胺合成反应不同的是可将γ -谷氨酰基转移于适当的受体，具有如羟胺的γ -谷氨酰基转移酶的活性。广泛分布于微生物和高等动植物中，除了可供给谷氨酰氨外，还有与谷氨酸合成酶偶联向α -氨基酸供给氨基的重要功能。大肠杆菌和枯草菌的酶是由分子量为 5万的单一的 12个亚基
抗体的检测外，还应该用与其抗原成分相关的其它抗原进行交叉试验，方法可用ELISA、IFA法。例如：①制备抗黑色素瘤细胞的McAb，除用黑色素瘤细胞反应外，还应该用其它脏器的肿瘤细胞和正常细胞进行交叉反应，以便挑选肿瘤特异性或肿瘤相关抗原的单克隆抗体。②制备抗重组的细胞因子的单克隆抗体，应首先考虑是否与表达菌株的蛋白有交叉反应，其次是与其它细胞因子间有无交叉。 2．McAb的Ig类与亚类的鉴定 一般在用酶标或荧光素标记的第二抗体进行筛选时已经基本上确定了抗体的Ig类型
免疫系统受抗原刺激后，B细胞转化为浆细胞，由浆细胞产生与抗原发生特异性结合的球蛋白，这类球蛋白称为抗体(antibody,Ab)。 免疫球蛋白(immunoglobulim,Ig)是指具有抗体活性或化学结构与抗体相似的球蛋白。 免疫球蛋白普遍存在于哺乳动物和人类的血液、组织液和外分泌中。人体的免疫球蛋白主要有五类：IgM、IgA、IgG、IgD、IgE。 (一) 抗体结构 抗体是具有4条多肽链的对称
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