- ￥1350 - 3000
- Proteintech
- 55100-1-AP
- 2025年10月13日
- WB, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
None
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, ELISA
- 靶点：
SPI1
- 抗体英文名：
SPI1 Polyclonal antibody
- 抗体名：
SPI1 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HepG2 cells, RAW264.7, mouse liver tissue, rat liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55100-1-AP targets SPI1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | spleen focus forming virus (SFFV) proviral integration oncogene spi1 |
| 别名 | 31 kDa transforming protein, OF, PU.1, SFPI1, SPI 1, SPI A, SPI1, Transcription factor PU.1 |
| 计算分子量 | 31 kDa |
| 观测分子量 | 31-42 kDa |
| GenBank蛋白编号 | NM_003120 |
| 基因名称 | SPI1 |
| Gene ID (NCBI) | 6688 |
| ENSEMBL Gene ID | ENSG00000066336 |
| RRID | AB_10858486 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P17947 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SPI1 binds to the PU-box which is a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. SPI1 is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. It also binds RNA and may modulate pre-mRNA splicing. The antibody is specific to SPI1. SPI1 can be detected 37-42 kDa (PMID: 28362429, 31586032, 28586009).
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文献和实验| Species | Application | Title |
|---|---|---|
| IF | Mucosal Immunol IL-33/ST2/IL-9/IL-9R signaling disrupts ocular surface barrier in allergic inflammation.Authors - Jiaoyue Hu | |
| mouse | WB | Sci Rep Specific immunotherapy in combination with Clostridium butyricum inhibits allergic inflammation in the mouse intestine.Authors - Yanhong Shi |
| human | WB | J Leukoc Biol miR-199a-5p inhibits monocyte/macrophage differentiation by targeting the activin A type 1B receptor gene and finally reducing C/EBPα expression.Authors - Hai-Shuang Lin
|
| human | WB | Med Oncol PU.1 negatively regulates tumorigenesis in non-small-cell lung cancerAuthors - Xiaoxiao Liu |
| human | WB | Exp Cell Res An innovative immunotherapeutic strategy for rheumatoid arthritis: Selectively suppressing angiogenesis and osteoclast differentiation by fully human antibody targeting thymocyte antigen-1Authors - Xuanxuan Hu |
| human | WB | Cell Rep A CLIC1 network coordinates matrix stiffness and the Warburg effect to promote tumor growth in pancreatic cancerAuthors - Jia-Hao Zheng |
14-3-3 sigma 14-3-3 sigma亚硫酸钠修饰的DNA 14-3-3 zeta c-Fos FosB Fra-1 Fra-2 c-Jun JunB JunD Fibronectin PAI-1 M16006 PAI-1 XM009352 GM-CSF FHIT IL-4 CD2AP real-time RT-PCR CCAAT/enhancer binding protein (C/EBP)：alpha / beta / delta
因此，综合来说，EarthOx的GST标签抗体 （货号#E022040）是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时，我们也提供其他品牌的GST标签抗体，如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody（货号#10000-0-AP）以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体，请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
实验，请根据说明书列出已验证的种属来选择适合你实验的抗体。 （4）样本蛋白的结构性质。了解样本蛋白的结构性质有助于选择最合适的抗体，待测样本蛋白的结构域和样本在提取和处理过程中是否会变性，蛋白空间构象的改变，会影响抗体的免疫亲和反应。 （5）单多克隆抗体的选择。市面上的抗体还是以多克隆抗体为主，一般单克隆抗体特异性强，但亲和力相对小，检测抗原灵敏度相对就低；而多克隆抗体特异性稍弱，但抗体的亲和力强，灵敏度高，但易出现非特异性染色（可以通过封闭等避免
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