相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
None
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
mouse
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Rabbit
- 应用范围:
WB, IHC, ELISA
- 靶点:
Marcks
- 抗体英文名:
Marcks Polyclonal antibody
- 抗体名:
Marcks Polyclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2200.0 |
| 规格: | 150ul | 产品价格: | ¥3000.0 |
经过测试的应用
| Positive WB detected in | mouse brain tissue |
| Positive IHC detected in | mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10004-2-Ig targets Marcks in WB, IHC, IF, IP, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, IHC, ELISA |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | mouse |
| 文献引用反应性 | mouse, rat, bovine |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | myristoylated alanine rich protein kinase C substrate |
| 别名 | Macs, Marcks, PKCSL |
| 计算分子量 | 39 kDa |
| 观测分子量 | 88 kDa |
| GenBank蛋白编号 | BC046601 |
| 基因名称 | Marcks |
| Gene ID (NCBI) | 17118 |
| RRID | AB_2140311 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P26645 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MARCKS is a member of MARCKS family of PKC substrate and it is also a widely accepted indicator of PKC activation. It shows a ubiquitous tissue distribution. The calculated molecular weight of MARCKS is 39 kDa and its apparent molecular weights were found at 68 kDa to 90 kDa in different species. This protein is a known to regulated cytoskeleton remodeling and membrane recycling. The anti-MARCKS antibody is raised against purified MARCKS protein from mouse brain using a method similar to that previously reported for MacMARCKS
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验| Species | Application | Title |
|---|---|---|
| mouse | IF | Dev Cell An integrated approach identifies the molecular underpinnings of murine anterior visceral endoderm migrationAuthors - Shifaan Thowfeequ |
| WB | Clin Transl Med Parkin regulates IGF2BP3 through ubiquitination in the tumourigenesis of cervical cancerAuthors - Xin Sun | |
| bovine | WB | Proc Natl Acad Sci U S A MARCKS protein mediates hydrogen peroxide regulation of endothelial permeability.Authors - Jin Benjamin Y BY |
| mouse | IF | Curr Biol Stretch triggers microtubule stabilization and MARCKS-dependent membrane incorporation in the shaft of embryonic axonsAuthors - Sara C Sousa |
| mouse | IF | J Cell Sci Dynamic adhesions and MARCKS in melanoma cells.Authors - Estrada-Bernal Adriana A |
| WB,IP | Carcinogenesis GRB-7 facilitates HER-2/Neu-mediated signal transduction and tumor formation.Authors - Bai Tao T |
一、多克隆抗体的概念抗原刺激机体,产生免疫学反应,由机体的浆细胞合成并分泌的与抗原有特异性结合能力的一组球蛋白,这就是免疫球蛋白,这种与抗原有特异性结合能力的免疫球蛋白就是抗体。抗原通常是由多个抗原决定簇组成的,由一种抗原决定簇刺激机体,由一个B淋巴细胞接受该抗原所产生的抗体称之为单克隆抗体(Monclone antibody)。由多种抗原决定簇刺激机体,相应地就产生各种各样的单克隆抗体,这些单克隆抗体混杂在一起就是多克隆抗体,机体内所产生的抗体就是多克隆抗体;除了抗原决定簇的多样性以外,同样
该法可用于分离提取人、兔、小鼠血清中的Ig及杂交瘤诱生的腹水和培养上清液中McAb。 一、材料和试剂 1. 正辛酸。 2. 60mmol/L,pH4.0醋酸缓冲液,PBS。 3. 透析袋。 4. 高速低温离心机。 二、操作步骤 1. 将待提取样品用60mmol/L,pH4.0醋酸缓冲液稀释3~4倍,调pH至4.5,对含脂质较高的标本可采用二氧化硅粉或玻璃纤维吸附法除去脂质。 2. 在室温下边搅拌边缓慢加入正辛酸,按每ml样品中加25μl,若样品体积
多克隆抗体技术(Polyclone antibodies preparation technique)
隆抗体混杂在一起就是多克隆抗体,机体内所产生的抗体就是多克隆抗体;除了抗原决定簇的多样性以外,同样一类抗原决定簇,也可刺激机体产生IgG、IgM、IgA、IgE和IgD等五类抗体。 (二)免疫 动物 供免疫用的动物主要是哺乳动物和禽类,常选择家兔、绵羊、山羊、马、骡和豚鼠及小鼠等。动物的选择常根据抗体的用途和量来决定,也与抗原的性质有关。如要获得大量的抗体,多采用大动物;如要是获得直接标记诊断的抗体,则直接采用本动物;如要获得间接的标记诊断用抗体,则必须用异源动物制备抗体;如果难以
技术资料暂无技术资料 索取技术资料











