- ￥1350 - 3000
- Proteintech
- 28030-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, ELISA
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human ATP1A3 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, ELISA
- 靶点：
ATP1A3 (middle)
- 抗体英文名：
ATP1A3 (middle) Polyclonal antibody
- 抗体名：
ATP1A3 (middle) Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HeLa cells, SH-SY5Y cells, SKOV-3 cells, U-87 MG cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28030-1-AP targets ATP1A3 (middle) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA |
| 文献引用应用 | WB, IF |
| 经测试反应性 | Human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag27705 Product name: Recombinant human ATP1A3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 378-580 aa of BC015566 Sequence: VAHMWFDNQIHEADTTEDQSGTSFDKSSHTWVALSHIAGLCNRAVFKGGQDNIPVLKRDVAGDASESALLKCIELSSGSVKLMRERNKKVAEIPFNSTNKYQLSIHETEDPNDNRYLLVMKGAPERILDRCSTILLQGKEQPLDEEMKEAFQNAYLELGGLGERVLGFCHYYLPEEQFPKGFAFDCDDVNFTTDNLCFVGLMS |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ATPase, Na+/K+ transporting, alpha 3 polypeptide |
| 别名 | ATP1A3, ATP1A3 (middle), DYT12, RDP, Sodium pump subunit alpha 3 |
| 计算分子量 | 113 kDa |
| 观测分子量 | 113 kDa |
| GenBank蛋白编号 | BC015566 |
| 基因名称 | ATP1A3 |
| Gene ID (NCBI) | 478 |
| RRID | AB_2881041 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P13637 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATP1A3 participates in the catalyticing hydrolysis of ATP and the exchanging of sodium and potassium ions across plasma membrane. The catalyticing activity mode is ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In). It has been published that the neurologic disorders rapid-onset dystonia-parkionsonism (RDP), alternating hemiplegia of childhood (ACH) and CAPOS syndrome (cerebellar ataxia, areflexia, pes cavus, optic atrophy and sensorineural hearing loss) are all related with the mutation of ATP1A3. There are other reports suggest that early life epilepsy and episodic apnea revealing are potentially associated with the mutation of ATP1A3 as a result of impairment of Na+/K+ homeostasis. This antibody is generated agaist the middle region (378-580aa) of ATP1A3 and detects 113 kDa protein in SDS-PAGE.(PMID: 30097153, 20301294, 29922587)
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB, IF | Sci Adv De novo ATP1A3 variants cause polymicrogyria.Authors - Satoko Miyatake |
| human | WB | Nat Commun Homophilic ATP1A1 binding induces activin A secretion to promote EMT of tumor cells and myofibroblast activation.Authors - Yi-Ing Chen |
Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
production. Following an 8 hour incubation, the filters are marked, removed, washed, blocked and incubated with the AP-fusion protein. Then, the filters are washed again and AP substrate (NBT/BCIP) is added indicating the positive plaques. These positive
from Biorad. Store in fridge.IV. 20% SDS (some people filter)V. 10% w/v ammonium persulfate, make fresh every month and store in fridge. Note: old bottles of dry AP can go bad.VI. N,N,N',N'-tetramethylethylene diamine (TEMED). Buy it and store in fridge.VII
因此，综合来说，EarthOx的GST标签抗体 （货号#E022040）是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时，我们也提供其他品牌的GST标签抗体，如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody（货号#10000-0-AP）以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体，请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
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