- ￥1350 - 3000
- Proteintech
- 27155-1-AP
- 2025年10月13日
- WB, IF/ICC, IF-P, IP, ELISA
- Rabbit
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human Caspase 7 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IF/ICC, IF-P, IP, ELISA
- 靶点：
Caspase 7
- 抗体英文名：
Caspase 7 Polyclonal antibody
- 抗体名：
Caspase 7 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | Jurkat cells, HEK-293 cells, LNCaP cells, PC-3 cells, MCF-7 cells, mouse liver tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27155-1-AP targets Caspase 7 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, IF-P, IP, ELISA |
| 文献引用应用 | WB, IHC, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat, pig, bovine |
| 免疫原 |
CatNo: Ag25904 Product name: Recombinant human Caspase 7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-100 aa of BC015799 Sequence: MADEQGCIEEQGVEDSANEDSVDAKPDRSSFVPSLFSKKKKNVTMRSIKTTRDRVPTYQYNMNFEKLGKCIIINNKNFDKVTGMGVRNGTDKDAEALFKC |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | caspase 7, apoptosis-related cysteine peptidase |
| 别名 | CASP7, Apoptotic protease Mch 3, Apoptotic protease Mch-3, CASP 7, CASP-7 |
| 计算分子量 | 303 aa, 34 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC015799 |
| 基因名称 | Caspase 7 |
| Gene ID (NCBI) | 840 |
| RRID | AB_2880779 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P55210 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Caspase 7(CASP7), like caspases 3 and 6, contains a short prodomain and, upon apoptotic induction, the 35 kDa proform is converted into a 32 kDa intermediate or preactive form which is further processed into two active subunits consisting of the p20 or large (18 kDa) subunit and the p10 or small (11 kDa) subunit and it is present in the brain, which is up-regulated and activated after traumatic injury(PMID:15953353). Caspase-7 is classified as a member of the subgroup of cysteine proteases most related to the Caenorhabditis elegans factor CED-3, which also includes caspase-3, -6, and -9(PMID:9426061). The protein is involved in the activation cascade of caspases responsible for apoptosis execution.
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文献和实验| Species | Application | Title |
|---|---|---|
| rat | WB | Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse modelAuthors - Mengyan Hu |
| human | WB | J Pineal Res Melatonin synergizes the chemotherapeutic effect of 5-fluorouracil in colon cancer by suppressing PI3K/AKT and NF-κB/iNOS signaling pathways.Authors - Yue Gao |
| human | WB | Cell Rep Inhibiting G6PD by quercetin promotes degradation of EGFR T790M mutationAuthors - Zehe Ge |
| human | WB | Front Immunol Identification of molecular subtypes based on PANoptosis-related genes and construction of a signature for predicting the prognosis and response to immunotherapy response in hepatocellular carcinomaAuthors - Jinfeng Zhu |
| human | WB | Phytother Res Targeting HSP90 with picropodophyllin suppresses gastric cancer tumorigenesis by disrupting the association of HSP90 and AKTAuthors - Xiaoli Li |
| human | WB | Cell Death Discov IDO1 inhibits ferroptosis by regulating FTO-mediated m6A methylation and SLC7A11 mRNA stability during glioblastoma progressionAuthors - Qianting Tian |
的Caspase-3及对底物多聚（ADP-核糖）聚合酶[poly（ADP-ribose）polymerase,PARP]等的裂解。方法：收集细胞→PBS洗涤→抽提细胞裂解液→蛋白定量→SDS-PAGE电泳→硝酸纤维素膜或PVDF膜转移→5%脱脂奶粉封闭，室温1.5~2h或4°C过夜→Caspase-3多抗或单抗室温反应1~2h或4°C过夜→TBS-T（含0.05% Tween 20的TBS）洗3次，5~10min/次→HRP-标记的羊抗鼠IgG或AP标记的羊抗鼠IgG室温反应1~2h→ TBS-T洗3次
的Caspase-3及对底物多聚（ADP-核糖）聚合酶[poly（ADP-ribose）polymerase，PARP]等的裂解。方法： 收集细胞→PBS洗涤→抽提细胞裂解液→蛋白定量→SDS-PAGE电泳→硝酸纤维素膜或PVDF膜转移→5%脱脂奶粉封闭，室温1.5~2h或4°C过夜→Caspase-3多抗或单抗室温反应1~2h或4°C过夜→TBS-T（含0.05% Tween 20的TBS）洗3次，5~10min/次→HRP-标记的羊抗鼠IgG或AP标记的羊抗鼠IgG室温反应1~2h→ TBS-T洗3次
。Smac前体在胞质中合成，继而转运到线粒体。成熟型的Smac通过N端的Ala―Val―Pro―Ile(AVPl)序列与细胞内的凋亡抑制蛋白(inhibitorofapoptosis protein，IAP)结合，后者是内源性的caspase抑制剂。1AP与caspase―9或caspase―3结合后将其灭活，从而抑制凋亡的发生。Smac与IAP的结合后，caspase―9和caspase―3释放，然后促发凋亡。 (二)caspase非依赖通路 尽管caspase是凋亡信号起始
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