- ￥1350 - 3000
- Proteintech
- 23660-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human ACTA1 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
- 靶点：
Alpha Actin
- 抗体英文名：
Alpha Actin Polyclonal antibody
- 抗体名：
Alpha Actin Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human colon tissue, human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue, mouse skeletal muscle tissue |
| Positive IF/ICC detected in | H9C2 cells |
| Positive FC (Intra) detected in | C2C12 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23660-1-AP targets Alpha Actin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 |
CatNo: Ag19151 Product name: Recombinant human ACTA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-377 aa of BC012597 Sequence: MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | actin, alpha 1, skeletal muscle |
| 别名 | ACTA1, α actin, ACTA, ACTA1 skeletal Muscle Actin, Actin alpha skeletal muscle |
| 计算分子量 | 42 kDa |
| 观测分子量 | 39-42 kDaa |
| GenBank蛋白编号 | BC012597 |
| 基因名称 | ACTA1 |
| Gene ID (NCBI) | 58 |
| RRID | AB_2879307 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | P68133 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Cell Metab QDPR deficiency drives immune suppression in pancreatic cancerAuthors - Ji Liu |
| mouse,human | WB | Nat Commun Overcoming therapeutic resistance in oncolytic herpes virotherapy by targeting IGF2BP3-induced NETosis in malignant gliomaAuthors - Weiwei Dai |
| WB | J Exp Med Tumor cell-intrinsic Piezo2 drives radioresistance by impairing CD8+ T cell stemness maintenanceAuthors - Naijun Miao | |
| mouse | WB | Acta Pharm Sin B A novel SIRT6 activator ameliorates neuroinflammation and ischemic brain injury via EZH2/FOXC1 axis.Authors - Tailin He |
Redox Biol Endothelial H2S-AMPK dysfunction upregulates the angiocrine factor PAI-1 and contributes to lung fibrosisAuthors - Xiangqi Chen | ||
| mouse | WB | Transl Res Different roles of BAG3 in cardiac physiological hypertrophy and pathological remodeling.Authors - Pengyu Jia |
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
and sensitive to changes in both the chemical and mechanical environment. Optimal conditions for fluorescence of proteins of the actin and tubulin cytoskeleton are based on: preserving cell structure; properties of individual cytoskeletal proteins
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells Actin: Louise Cramer Tubulin: Arshad Desai General Strategy
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