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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human ER protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
human, mouse, rat
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Rabbit
- 应用范围:
WB, IHC, ELISA
- 靶点:
ER
- 抗体英文名:
ER Polyclonal antibody
- 抗体名:
ER Polyclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2200.0 |
| 规格: | 150ul | 产品价格: | ¥3000.0 |
经过测试的应用
| Positive WB detected in | HeLa cells, mouse testis tissue, NIH/3T3 cells, rat testis tissue, MCF-7 cells |
| Positive IHC detected in | human cervical cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21244-1-AP targets ER in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, bovine, goat |
| 免疫原 |
CatNo: Ag15738 Product name: Recombinant human ER protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 65-280 aa of BC128573 Sequence: AAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEV |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | estrogen receptor 1 |
| 别名 | ESR1, ER alpha, Era, ER-alpha, ERα |
| 计算分子量 | 595 aa, 66 kDa |
| 观测分子量 | 65-70 kDa |
| GenBank蛋白编号 | BC128573 |
| 基因名称 | ER |
| Gene ID (NCBI) | 2099 |
| RRID | AB_11042600 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P03372 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The estrogen receptor (ESR, ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. ESR1, also known as ESR or NR3A1, belongs to the nuclear hormone receptor family and NR3 subfamily. It is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. ESR1 can activate the transcriptional activity of TFF1.[PMID: 11731608,10970861]
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- 作者
- 内容
- 询问日期
文献和实验| Species | Application | Title |
|---|---|---|
| mouse | WB | Mol Cell PGC-1α senses the CBC of pre-mRNA to dictate the fate of promoter-proximally paused RNAPIIAuthors - Xavier Rambout |
| mouse | WB | J Biomed Sci Dioscin promotes osteoblastic proliferation and differentiation via Lrp5 and ER pathway in mouse and human osteoblast-like cell lines.Authors - Chunfang Zhang |
| human | IHC | Theranostics Single-cell analyses reveal evolution mimicry during the specification of breast cancer subtypeAuthors - Zhi-Jie Gao |
| mouse | WB,IHC | J Hazard Mater Cyanidin-3-O-glucoside ameliorates cadmium induced uterine epithelium proliferation in mice.Authors - Dacheng Yang |
| human | WB | J Med Chem Discovery of Thiochroman Derivatives as Potent, Oral Selective Estrogen Receptor Degraders and Antagonists for the Treatment of Endocrine-Resistant Breast CancerAuthors - Dan Zhang |
| human | WB | J Environ Sci (China) The effects of 17β-trenbolone and bisphenol A on sexual behavior and social dominance via the hypothalamic-pituitary-gonadal axis in male miceAuthors - Xiang Zuo |
Immunity:北大蒋争凡课题组发现 STING 后高尔基体囊泡转运的重要性及机制
4KB 与 ARMH3 互作的关键区域(6 个氨基酸残基)都会显著削弱 cGAS-STING 通路的激活。这些结果表明 ARMH3 招募 PI4KB 对于 STING 的激活十分关键。 更重要的是,细胞内敲除 ARMH3 或者原位删除 PI4KB 结合 ARMH3 所需的 6 个氨基酸,STING 在上游通路活化后几乎呈现完全的内质网定位,暗示如果 2'3'-cGAMP 结合后被转运到高尔基体的 STING 无法被继续转运到后高尔基体囊泡,将会被细胞内 COPI/AP1 介导的从高尔基体向内质网的逆向转运运回
因此,综合来说,EarthOx的GST标签抗体 (货号#E022040)是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时,我们也提供其他品牌的GST标签抗体,如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody(货号#10000-0-AP)以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体,请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
-1 信号,另一方面激活具有肿瘤抑制功能的 SHRs 信号。免疫组化分析进一步证实,单独禁食或联合 TMX 均显著增加了 GR 的核定位,这是 GR 激活的典型标志,而 ERα 和 PR 的亚细胞定位则未受影响。ChIP-seq 实验直接证明了禁食诱导了 GR 和 PR 的染色质结合显著增强,而 JUN 在新获得的 H3K27ac 位点上并无 occupancy,说明禁食确实实现了从 AP-1 驱动到 GR/PR 驱动的转录程序转换。 图 3. 禁食增强 GR 和 PR 染色质结合并降低
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