- ￥1350 - 3000
- Proteintech
- 19762-1-AP
- 2025年10月13日
- IHC, IF/ICC, IF-P, ELISA
- Rabbit
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
None
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
IHC, IF/ICC, IF-P, ELISA
- 靶点：
GPR50
- 抗体英文名：
GPR50 Polyclonal antibody
- 抗体名：
GPR50 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:100-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19762-1-AP targets GPR50 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, IF/ICC, IF-P, ELISA |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | G protein-coupled receptor 50 |
| 别名 | Melatonin related receptor, Mel1c, H9, G protein-coupled receptor 50, G protein coupled receptor 50 |
| 计算分子量 | 67 kDa |
| GenBank蛋白编号 | NM_004224 |
| 基因名称 | GPR50 |
| Gene ID (NCBI) | 9248 |
| RRID | AB_2878603 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13585 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPR50, also known as Melatonin-related receptor or H9, is a member of the G-protein coupled receptor 1 family. It is closely related to the G protein-coupled melatonin receptor family and has been demonstrated as the mammalian ortholog of Mel1c receptor (PMID: 8647286; 18400093). GPR50 does not bind melatonin. It is able to heterodimerize with both the MT1 (Mel1a) and MT2 (Mel1b) melatonin receptor subtypes. While GPR50 has no effect on MT2 function, GPR50 prevented MT1 from both binding melatonin and coupling to G proteins. GPR50 is involved in neuroendocrine function and plays a role in the regulation of energy metabolism (PMID: 8647286; 17957037).
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Nat Cell Biol Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins.Authors - Boris Simonetti |
| mouse | WB | J Cereb Blood Flow Metab Agomelatine promotes differentiation of oligodendrocyte precursor cells and preserves white matter integrity after cerebral ischemic strokeAuthors - Shisi Wang |
| mouse | WB,IP | Transl Stroke Res Melatonin Offers Dual-Phase Protection to Brain Vessel Endothelial Cells in Prolonged Cerebral Ischemia-Recanalization Through Ameliorating ER Stress and Resolving Refractory Stress GranuleAuthors - Danli Lu |
| human | IF | Front Pharmacol An Improved Genetically Encoded Fluorescent cAMP Indicator for Sensitive cAMP Imaging and Fast Drug Screening.Authors - Wenfeng Liu
|
| human | WB | Am J Chin Med Tetramethylpyrazine Attenuated Sevoflurane-Induced Neurotoxicity by Enhancing Autophagy through GPR50/CREB Pathway in SH-SY5Y Cells.Authors - Lili Xu
|
| mouse | WB | FEBS Open Bio Orphan receptor GPR50 attenuates inflammation and insulin signaling in 3T3-L1 preadipocytesAuthors - Zhenyu Yao
|
因此，综合来说，EarthOx的GST标签抗体 （货号#E022040）是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时，我们也提供其他品牌的GST标签抗体，如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody（货号#10000-0-AP）以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体，请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
实验，请根据说明书列出已验证的种属来选择适合你实验的抗体。 （4）样本蛋白的结构性质。了解样本蛋白的结构性质有助于选择最合适的抗体，待测样本蛋白的结构域和样本在提取和处理过程中是否会变性，蛋白空间构象的改变，会影响抗体的免疫亲和反应。 （5）单多克隆抗体的选择。市面上的抗体还是以多克隆抗体为主，一般单克隆抗体特异性强，但亲和力相对小，检测抗原灵敏度相对就低；而多克隆抗体特异性稍弱，但抗体的亲和力强，灵敏度高，但易出现非特异性染色（可以通过封闭等避免
发生，最好在特异性抗体处理切片之前，选择与二抗种属相同的非免疫血清封闭电荷，阻止一抗与之结合，抑制非特异性背景着色。常用方法是用2%～10%羊血清或2%～5%牛血清白蛋白在室温下作用10~30分钟。但应注意此种结合不牢固，所以最好不要冲洗，倾去余液直接加一抗。SABC或SP免疫组织化学试剂盒中常配有非免疫血清。 7．第一抗体孵育 滴加特异性一抗于切片上，4℃孵育24～48小时，或室温孵育过夜，也可在37~C孵育1～2小时。之后，用PBS冲洗3次，每次5分钟。一抗分为多克隆抗体和单克
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